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Navigate our extensive portfolio of HPLC and UHPLC systems by using the system properties on the left to filter the list and discover which liquid chromatography system best fits your requirements. Build your ideal HPLC or UHPLC with our step-by-step configurator
Alexa Fluor Oligonucleotide Amine Labeling Kits. The Invitrogen Alexa Fluor Oligonucleotide Amine Labeling Kits ( Molecular Probes nucleic acid labeling kits—Table 8.6) provide the reagents required for labeling synthetic oligonucleotides that have amine groups incorporated at their 5'- or 3'-terminus.
Oct 30, 2016 · This ultra HPLC system is designed to be both a HPLC and high-end UHPLC system in a single flow path, giving you robust and excellent performance no matter what application you are running while seamlessly integrating into your existing laboratory environment.
Use only HPLC grade solvents, salts, ion pair reagents, and base and acid modifiers. Cleaning lower quality solvents is time consuming, and trace levels of contaminants often remain. These trace contaminants can cause problems when you use a high sensitivity ultraviolet or fluorescence detector.
High Performance Liquid Chromatography (HPLC) is a process of separating components in a liquid mixture. A liquid sample is injected into a stream of solvent ( mobile phase) flowing through a column packed with a separation medium ( stationary phase ).
Pre-column Derivatization Method (pre-label method) n pre-column derivatization, the amino acids are derivertized before injection, and then the reaction products are separated and detected. This concept is illustrated on the right. The "labels" shown indicate the derivatizing reagent. The advantages of this method are as follows:
Oct 30, 2016 · This ultra HPLC system is designed to be both a HPLC and high-end UHPLC system in a single flow path, giving you robust and excellent performance no matter what application you are running while seamlessly integrating into your existing laboratory environment.
Finally, although an HPLC is capable of separating and analyzing complex mixtures, an analysis may still be difficult if the number of constituents exceeds the column’s peak capacity. 2. One advantage of an HPLC analysis is that a loop injector often eliminates the need for an internal standard.
Description. Offering excellent linearity and noise performance across a wide operating range, Thermo Scientific Vanquish Variable Wavelength Detectors provide versatile, robust, high-performance UV-Vis absorption detection. With up to 250 Hz data collection rate, the detector meets both current and future HPLC and UHPLC detection challenges.
Jun 28, 2021 · The HPLC columns have fixed lengths, diameter, and also the particle size of the stationary phase to choose from based on our requirement. The longer columns are used for the separation of the complex mixture with many components. While shorter columns are used to separate less complex mixtures.
Thermo Scientific Viper Fingertight fittings, for HPLC and UHPLC systems, seal at the tip of the tubing and virtually eliminate dead volume. These reusable fittings make it easier to plumb your HPLC or UHPLC system without any tools for reproducible leak-free connections to pressures up to 1500 bar. Conventional fittings
Sep 5, 2022 · High-performance liquid chromatography (HPLC) is a technique in analytical chemistry used to separate the components in a mixture, and to identify and quantify each component. It was initially discovered as an analytical technique in the early twentieth century and was first used to separate colored compounds.
Purge pump at high flow rate (e.g., 5-10 mL/min.), prime system if necessary. (Prime each pump head separately.) If system has check valve, loosen valve to allow air to escape. If problem persists, flush system with 100% methanol or isopropanol. If problem still persists, contact system manufacturer.
High-performance liquid chromatography (HPLC) is a broad analytical chemistry technique used to separate compounds in a chemical mixture. These separations utilize the pressure-driven flow of a mobile phase through a column packed with a stationary phase. Example of an HPLC chromatogram.
This application note clearly demonstrates the ability to obtain high-quality, DMB-labeled sialic acid analyses on geometrically scaled HPLC, UHPLC, and UPLC column configurations containing same Waters BEH C 18 , 130Å reversed-phase-based chemistry on 3.5 μm (HPLC), 2.5 μm (UHPLC), and 1.7 μm (UPLC) particles.