Aug 01, 2009 · 2.4. Quantitative analysis by gas chromatography (GC) The GC analyses were performed on a Hewlett–Packard Series II gas chromatograph (Aijiren Technologies, Palo Alto, CA, USA) equipped with a flame ionization detector (FID). The polymer OV-215 was dissolved in acetone or ethyl acetate.
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Jul 01, 2003 · To each vial, 1 ml of ethyl acetate (99.8% pure HPLC Grade Sigma Aldrich, UK) containing a 0.01% v/v triethylamine buffer (99% pure, minimum Sigma Aldrich, UK) and an internal standard of 7.5 μl of quinoline (98% pure HPLC Grade Sigma Aldrich, UK) was added .
Page 2 of 7 Product of USA Solution Standard Verification Standard Solution Assay Parameters Calibration Curve Analysis Method: HPLC/UV Calibration Curve: Linear Regression Column: Betasil Phenyl, 4.6 x 150 mm Number of Points: 4 Mobile Phase: Acetonitrile::10 mM Potassium phosphate buffer (70::30) Linearity (r) : Flow Rate: 1.5 mL/min
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The organic layer is decanted and placed into a 2 mL GC/LC sample vial. Depending on the detection scheme used and the desired reporting limits, a 2-10X concentration/dilution of the extract may be required to achieve the necessary sensitivity. Step 10: Preservation & Analysis