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Aug 25, 2020 · 11. Transfer 90 µl of the supernatant to glass HPLC vials. 12. Run all the samples and standards on HPLC. HPLC program (also see Strategic Planning and Basic Protocol 5) The analysis of adenosines is performed by reversed‐phase HPLC on a Hyperclone C18 (ODS) column connected to an HPLC system (Dionex).
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The sample with writing was cut (0.5x1cm2) and placed in a screw cap UPLC sample vial with 500 μL to which solvent (1.25% acetic acid: CH 3CN = 70 : 30 v/v%) was added. The vial was gently rotated with a Clay Adams Nutator Mixer for one hour. The solution was transferred to a screw cap UPLC max recovery sample vial for analysis.
----- table of contents list of acronyms v 1.0 introduction 1 2.0 environmental management in small laboratories 4 2.1 environmental management systems 4 2.2 pollution prevention and waste minimization 6 2.3 environmental training 7 2.4 information management and recordkeeping 9 3.0 key environmental management issues 11 3.1 air emissions 11 3.2 water discharges 21 3.3 hazardous wastes 28 3.4
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Jan 01, 2003 · This mixture was heated at 170 °C in a reaction oven. The guanidine adducts of sugars were monitored by a fluorescence detector with excitation at 310 nm and emission at 415 nm. The flow rate of both the mobile phase and the guanidine reagent was 0.5 mL/min. A diagram of the present HPLC system is shown in Fig. 1. The mannose concentration was
Nalgene® cryogenic vials capacity 2.0 mL, sterile, External threads; find null-V5007 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
Sep 05, 2020 · Cell culture media used was RPMI 1640 with l-glutamine with MOPS buffer obtained from Capricorn Scientific (Ebsdorfergrund, Germany), Lonza (Aubergenville Cedex, France) and RPMI-1640 medium with l-glutamine and without sodium bicarbonate (Sigma, Saint Quentin Fallavier, France) with addition of 1% glucose and 0.0825 mol/L MOPS (Sigma, Saint
Tubes and vials are supplied in a range of heights, diameters, wall thicknesses, bottom shapes (round or flat), rim and closure styles in capacities from a few microlitres up to 25mL. Closures can be simple stoppers, screw-tops, push-tops, snap-tops or crimp-tops.
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Jul 15, 2020 · Headspace screw top 20 mL clear vials and magnetic polytetrafluoroethylene (PTFE)/Sil screw caps for headspace vials, 18 mm thread, were purchased from Aijiren Technologies (Santa Clara, CA, USA). Water was obtained by means of the Milli-Q RG apparatus by Millipore (Millipore Intertech, Bedford, MA, USA) in our laboratory. 2.2. Biological material
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