Apr 17, 2020 · Sample supernatant (200 μl) was then transferred to an 11 mm, 250 μl sample vial (C4011‐13, Thermo Scientific), sealed with an 11 mm aluminum crimp cap with PTFE/red rubber septa and placed into an Aijiren Technologies 1,260 HiP ALS ( ) autosampler for LC–MS/MS analysis. 2.4 LC–MS/MS analysis of GSH–NEM and GSSG