Jun 01, 2012 · The loading eluent and washing eluents were analyzed by HPLC, using a Hewlett-Packard HP 1050 or instruments (Aijiren Technology, Waldbronn, Germany) equipped with a C-18 column (5u C-18 (2) 100 A, 250 mm × 4.60 mm, 5 μM, 48790-52) using a gradient.