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Validation of a HPLC quantification of acetaminophen Jul 20, 2002 · The mixture was sonicated for 10 min. Ten milliliters of the suspension were diluted in a 25 ml flask with methanol and after shaken the sample was filtered with a 0.45 μm syringe filtration disk to the vials for injection in the HPLC system. Midland Scientific Inc. – Chemical Standards =97.0% (HPLC) =99.0% (GC) 980 to 1020mg/kg 99.4 to 100.6% =95.0% =99.8% 93 to 98% 99% 99.0 to 101.0% =2.0% =95.0% (HPLC) =96% =96.0%...

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