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Standard Operating Procedure for High Performance Liquid Mar 30, 2012 · 7.3 All the solutions which are entering the HPLC System i.e. water, mobile phase, etc. must be filtered through 0.4 µ membrane filter paper, and properly degassed. 7.4 In case of mobile phase containing buffer, it is essential to give water washing to the column prior and after running the mobile phase. HPLC System Suitability: Which is the Right Match for My Lab? The II LC is an integrated system for ro...