2. Transfer tissue samples to a 2 ml v-bottom freezing vial (Catalog Number V5381) held at –20 C. Immediately proceed to step 3 to minimize any protein degradation. 3. Add 1.5 ml of the prepared Methanol Solution. Briefly vortex the sample (15–30 seconds) and place at –20 C. Allow the mixture to incubate for
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Sigma-Aldrich Online Catalog Product List: 2 mL. System Maintenance Alert: Due to planned maintenance of our internal systems, web functionality including order placement, price and availability checks and SDS display will not be available for Asia and several European countries from Saturday, November 7th at 2:30 CET until Sunday, November 8th at 7:00 AM CET.
After reconstitution, it can be stored at 2-8°C for one month, or frozen in aliquots at -70°C or -20°C for longer periods. Preparation Note This product was lyophilized from a 0.2 μm-filtered solution of phosphate buffered saline, at pH 7.4. It should be reconstituted by adding the contents of the vial to 1 mg/mL using 10 mM acetic acid.