Aug 11, 2020 · From this, 100 μl was transferred to LC vials, and 30 μl of each sample was pooled to a mixed QC sample. LC-HRMS was performed on a II ultra-high performance liquid chromatography (UHPLC) system coupled to a 6550 iFunnel quadrupole-time of flight (Q-TOF) mass spectrometer equipped with a dual AJS electrospray ionization source