Mar 01, 2022 · The extraction method was adapted from Ref. and consisted in shaking 1 g of dried and sieved (2 mm) soil in 2 mL of ACN for 1 h at 20 rpm on a vertical shaker at room temperature; followed by centrifugation (1882×g; 5 min, 20 °C) and filtration of the supernatant through 0.22 μm filters into HPLC glass vials.