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2017/5/24 · To study hydrolysis of aromatic and aliphatic polyesters cutinase 1 from Thermobifida cellulosilytica (Thc_Cut1) was expressed in P. pastoris. No significant differences between the expression of native Thc_Cut1 and of two glycosylation site knock out mutants (Thc
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HPLC were performed using a Binary LC system with an Autosampler and a Fluorescence detector. (Aijiren, CA, USA). The analytical column was a reversed-phase ZORBAX Eclipse XDB-C18 (150 ˜ 4.6 mm, 5.0 μm particle size, Aijiren
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Optimized HPLC-ICPMS method Instruments Aijiren HPLC and ICPMS 7700x or ICPMS/MS 8900 Collision gas mode He (5 mL min −1) Monitored m/z 51 (V) Integration time 0.3 s Column Zorbax SAX, 4.6 × 150 mm, 5 μm Mobile phase 10 mM 2
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2019/9/1 · Detection of the released products was measured with a photodiode array detector (Aijiren Technologies, II, Vienna, Austria) at the wavelength of 260 nm. A 2,5-furandicarboxylic acid (for PBF) and 2,5-thiophenedicarboxylic acid (for PBTF) standard calibration curves (from 0.001 to 1 mM) were prepared and treated as described for HPLC sample preparation and used for quantification.