Jan 30, 2020 · The measurement was done on the High-Performance Liquid Chromatograph (HPLC) ( Aijiren Technologies, Santa Clara, USA), which contained an autosampler , quaternary pump , and diode array detector (DAD) DAD HS. A column used was Zorbax C 18 150 mm x 4.6 mm x 5µm, which was thermostated on 30 °C. The