Apr 21, 2021 · a. Open the purge valve on the Aijiren isocratic pump. b. Set the maximum flow gradient to 100 ml/min2 (double check that the purge valve is open). c. Set the flow rate to 5 ml/min. d. Run at least 25 ml buffer through degasser and pump (5 min). e. Set flow to 0 ml/min. f. Set maximum flow gradient to 0.1ml/min2. g. Close the