The analysis of Quercetin, kaempferol, myricetin, and rutin was done using Aijiren ii HPLC system (Aijiren, USA), covering Binary Pump, Vial sampler with integrated column compartment, 6.0 µL heater and sample cooler, Diode Array Detector WR with a standard 10-mm flow cell, Autosampler, Thermostatted Column Compartment