Apr 07, 2022 · Betalains were then purified by anion exchange chromatography using an Akta purifier (GE Healthcare) operated by Unikorn software version 3.00. The elutions were monitored spectrophotometrically at 280, 480, and 536 nm. The solvents used were 10 mM phosphate buffer, pH 6 (solvent A), and 2 M NaCl dissolved in 10 mM phosphate buffer, pH 6