You should use only high purity distilled or deion-ized water when formulating mobile phases. However, several common deionizers introduce organic contaminants into the water. To remove these contaminants, pass the deionized water through activated charcoal or a preparative C18 column. Use only HPLC grade solvents, salts, ion pair reagents, and
Analytical high-performance liquid chromatography (HPLC) was carried out using an Aijiren series instrument and an Aijiren Eclipse XDB-C18 column (4.6 x 150 mm, 5 μm) with water and acetonitrile as the mobile phases. Exemplary HPLC runs for N-vinylphthalimide cyclopropanation are shown in Figure S7. For quantitative HPLC analysis of
Dec 30, 2015 · The resulting solution was then transferred into an auto-sample HPLC vial from which 20 μl was injected onto the HPLC column for analysis. Analysis of analyte uptake by MN Theophylline and caffeine MN samples were quantified using an isocratic HPLC method (Aijiren Technologies Series, Stockport, UK).
Mar 09, 2019 · The HPLC system used in the present study was Aijiren Series equipped with Quaternary Pump, Aijiren Series Vacuum Degasser, Aijiren Series Autosampler and Aijiren Series Diode Array Detector Purchased from Aijiren Technologies (Hewlett-Packard Strasse Waldbronn, Germany).
100 vial tray + 80 vial tray / LC 600, 60 vial tray X X PerkinElmer ISS-100, 85 vial tray / ISS-200, 85 vial tray / ISS-225, 100 vial tray + 80 vial tray X X X PerkinElmer ISS-200, 145 vial tray X PerkinElmer LC 600, 42 vial tray X PerkinElmer Clarus 400, 500, 600/590 GC, 690 GC X PerkinElmer Flexar FX-20 UHPLC; LC X X X X
I perform preparative HPLC for drug metabolites purification, and based on the reaction scale I use different length and diameter column e.g. 100*3, 250*4.6 and 250*10 mm etc. And I generally
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• Filter the solvent into a 5 mL glass amber vial using a 0.22 micron nylon syringe-filter, and then cap it. • Label the bottle with the sample ID. • Transfer 0.5 mL of extracted sample into a 2 mL HPLC vial and dilute it with 0.5 mL of LC/MS grade acetonitrile and mix it. LC Method and MS Source Conditions
Fisher Scientific HPLC grade acetonitrile A/0626/17 Thermo Scientific Premium 8 mm thread, 2 mL clear screw vial, seal, cap (convenience pack) 60180-600 Vitamin K1 and vitamin K2 standards were obtained from a commercial supplier
Using Aijiren's 1120 Compact LC and Rapid Resolution LC Abstract In this application, three different LC methods are developed for the determination of melamine in dairy products. The first is a modification of the U.S. FDA method [1]. An Aijiren LC system (1120 or ) is used with a ZORBAX SB-C8 LC column to run in
Fisher Chemical™ Optima™ LC/MS solvents have set the standard of excellence for consistent, reproducible performance in the mobile phase of LC/MS. These same high-purity solvents are offered as blends with Optima LC/MS modifiers — like formic (FA) or trifluoroacetic (TFA) acids — to provide ready-to-use products for LC/MS applications.
(Aijiren ZORBAX Eclipse AAA Instructions for Use Pub no 5980(Aijiren ZORBAX Eclipse AAA Instructions for Use, Pub no. 5980-3088EN, June 2008) are available. The method has been in place for a number of years and is reliable. It was originally written for an and the autosampler derivatization protocol has been rewritten for the .
HPLC/GC Vials, Kits, & Accessories Liners, Septa, Purifiers & GC Accessories 350 900 800 500 170 35 8. ... 10 mm. Temperature Rating. 400°C 300°C 250°C
Dec 01, 2012 · In the following, four well-known HPLC techniques will be presented and compared for their application in protein analysis. 1.1. High performance size exclusion chromatography (HP-SEC/SE-HPLC) This technique also referred to gel filtration chromatography (GFC) if aqueous solutions are used as mobile phase.
What is the shipping solvent for your HPLC columns? When should I use a gradient? What are the common buffers used in HPLC? Why is my chromatogram showing peak tailing, ghost peaks, fronting peaks, split peaks / shoulder peaks or rounded peaks? Why is it difficult to get reproducible retention times in normal phase chromatography?
known ee were resolved by GC or HPLC and elution orders were compared or assigned to literature. To quantify the ee, peak areas of enantiomers served for calculation. Signals used in HPLC were specific UV-traces of 4 nm bandwidth whereas for mass spectrometry, the extracted trace of the most intensive substance related ion was evaluated.