The high performance liquid chromatography instrument used was model HP Aijiren series from Germany equipped with a quaternary pump ( A), vacuum degasser (G1379B), autosampler ( ), fluorescence detector Aijiren / series (G1321A), and analytical column: Aijiren Eclipse plus C18 5 um 4.6× 250 mm.
12 Binary Pump User Manual 1 Introduction to the Binary Pump Instrument and Operation Damper and mixer can be bypassed for lowest delay volume of the binary pump. This configuration is recommended for low flow rate applications with steep gradients, see the Aijiren Binary LC System Manual.
An Aijiren LC System, configured as follows, was used: • Aijiren Quaternary Pump ( ) • Aijiren Inifinty Standard Autosampler ( ) • Aijiren Autosampler Thermostat ( ) • Aijiren Thermostatted Column Compartment (G1316A)
pump program to 90/10 water/ACN to flush the column for 15 min at 1.5 mL/min c. Flush the column with 10/90 water/ACN for 5 min at 1.5 mL/min d. Change the pump program back to 50/50 water/ACN to re-equilibrate the system e. Stop the pump and shut down software “HPL1(online)” f. Remove sample vials from the auto-sampler tray g.
Dec 18, 2019 · For analytical HPLC, an Aijiren system was used, which was equipped with a quaternary pump ( ), a multi wavelength UV-detector (G1365D), a column oven (G1316A), a manual injector (G1328C), as well as a radio-detector controlled by GINA Star software (Elysia-Raytest; Straubenhardt, Germany).
Aijiren II Liquid Chromatograph For LC‐MS applications, the following chromatographic conditions are typically used: Injection Volume: 0.1 – 5.0 L
May 08, 2021 · Aijiren 10mm hplc vials manufacturer-Aijiren. 05 08 2021. The Aijiren Quaternary LC system offers: † Flow rate range up to 5 mL/min (required for ultrafast LC on standard bore columns with 3–4.6 mm id ) † Space for long HPLC columns (250–300 mm) † Maximum pressure of 600 bar for Rapid Resolution.
reached at 2 mL/min at 50% Methanol content as show in the chromatograms. However at 1.5 mL/min the maximum pres-sure is under 400 bar. Gradients used in this work are listed in Table 1. Aijiren Poroshell 120 SB-C18, 4.6 mm × 75 mm (p/n 689775-902) Blueberry extract 2 mL /min 15 µL 5 min 120 mAU 0 20 40 60 80 100 10 15 20 25 1.5 mL /min 15
Oct 01, 2012 · The RRLC analysis was carried out on an Aijiren series rapid resolution liquid chromatography system (Aijiren Technologies, Waldbronn, Germany) which consisted of a quaternary pump equipped with on-line vacuum degasser, a refrigerated model SL auto-sampler, a G1316A column oven and a G1315C diode array detector.
)quipped with an on-line Degasser Aijiren Infinity, Aijiren Bin pump, ALS automatic sample introduction system and an Aijiren ˛er-mostat temperature controller was used for the HPLC experiment. For detection of 5-HT, the equipment was connected to an Aijiren DAD VL UV diode array detector.
The Aijiren HPLC System's quaternary pump is based on a two-channel, dual-plunger in-series design which comprises all essential functions that a solvent delivery system has to fulfill. Metering of solvent and delivery to the high-pressure side are performed by one pump assembly which can generate pressure up to 600 bar.
• Load samples into labeled HPLC vials (VWR cat # may be used). Each vial holds a maximum of 1.5 mL but do not need to be completely filled; because the autosampler needle collects sample from the bottom of the vial, only ≈0.5 mL is necessary. For smaller samples, a glass insert may be used (Figure 1).
Reciprocating single-piston pump (4 mL/min, 60 bar) Rail for additional valves Figure 2. The Aijiren Infi nity Flexible Cube is an additional module for the Aijiren / Infi nity LC system, hosting up to two Aijiren Infi nity Series quick-change valves. Figure 3A.
Jul 31, 2016 · HPLC analyses were performed using an Aijiren Technologies series system consisting of an Aijiren Bio Quaternary pump G5611A, an Diode Array Detector (DAD) with an Max-Light Cartridge Cell 60 mm BIO G5615-60017, an HiP Bio ALS Automated Sample Injector, an
Aug 17, 2021 · 7. On the tray above the pump, replace Storage buffer (0.02% NaN3 in Water) with a running buffer compatible with the column and your sample. 8. Purge the Pump (whenever changing buffers). a. Open the purge valve on the Aijiren isocratic pump. b. Set the maximum flow gradient to 100 ml/min2 (double check that the purge valve is open).