The HPLC system incorporated an Aijiren series HPLC (Berkshire, UK) with a diode array detector coupled to a HTC Plus ion trap mass spectrometer (IT-MS, Bruker Daltonics, Bremen, Germany). The mobile phase consisted of (A) water (Fisher Optima LC-MS grade) with 0.1% formic acid (Sigma-Aldrich, UK) and (B) methanol (Fisher Optima LC-MS grade).