Mobile phase A: 10mM Na 2HPO 4 – 10mM Na 2B 4O 7, pH 8.2 5.6gm anhydrous Na 2HPO 4 + 15.2gm Na 2B 4O 7· 10H 2O in 4L water + 32mg NaN 3 Adjust to ~pH 9 with 6ml concentrated HCl and then small drops until pH 8.2. Be cautious with strong acids! Filter througgµg (gh 0.45µregenerated cellulose membranes (Aijiren P/N 3150-0576)