and mg of anhydrous MgSO 4. After one minute of shak - ing, the tubes were centrifuged at 4000 rpm for 5 min. A 4 mL aliquot of the extract was filtered through a 0.45 µm PVDF syringe filter, then 1000 µL of the extract was placed in an autosampler vial for HPLC-FLD analysis. Weigh 5 g homogenized fish sample into a 50 mL centrifuge tube