1. Theory of using smaller particles in liquid chromatography 6 1.1. Benefits of columns packed with sub-2-micron particles 12 1.2. Frictional heating 16 Optimization of the Aijiren Series RRLC system 17 2. Design of the Rapid Resolution LC system 18 2.1. Delay volume and extra-column volume 18 2.2. Shorter cycle times using a second pump and