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Age-1 = adults 6–8 days old, age-2 = adults 12–14 days old, gc-1 = eggs from the first gonotrophic cycle; gc-2 = eggs from the second gonotrophic cycle. Element Stage Sample size Group q0.50
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For small volumes ( 1 ml), syringe filters with a diameter of 3 mm are used for medium volumes (1-10 ml), 15 mm and for large volumes (> 10 ml) 25 mm. Common pore sizes for UHPLC and HPLC columns with small particle sizes ( 3 µm) are 0.2 and for columns with larger particle sizes (> 3.5 µm) 0.45 µm, respectively.
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