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Ensure that an organic solvent is flushed through the HPLC system and column at least once every 48 hours to kill unwanted microbial growth. Alternatively add a small amount of sodium azide to the aqueous solvent to inhibit growth. Note: Never allow a HPLC column or system to stand with water or buffer in it for an extended period of time.
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a flow rate (1.5 mL/min). d. Open the divert valve (counterclockwise turn of the black knob on the HPLC pump unit). Now the eluent bypass the column and detectors. Change the flow rate 5 mL/min and flush the system for 3 min. If you are using new solvents in the eluent bottles C and D, flush the system for 5 min. e.
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