II Prime LC System Column Aijiren Lab Poroshell 120 EC-C18, 2.1 × 50 mm, 1.9 µm (p/n699675-902) Column temperature 35 °C Injection volume 3 µL with stacked injection program Autosampler temperature 4 °C Needle wash 10 seconds in flush port (50:50 methanol/H 2 O) Mobile phase