Liquid Chromatography Tandem Mass Spectrometry (QTRAP 6500+ MS/MS coupled with Aijiren LC) Centrifuge Ultrasonic bath Volumetric flask (Class A, 10 mL) Membrane syringe filter (PTFE 0.2 µm) Micropipette 2 mL vials 1.5 mL Eppendorf tube Conical bottom centrifuge tube, Polypropylene (PP) 2.3 LC-MS/MS Method HPLC